DNA purification

English What's DNA purification? DNA purification is defined as the process of isolation of DNA from samples using physical and chemical methods. Aims Purity (free from contamination and other major cell components such as proteins, carbohydrates, lipids, other nucleic acids, ..) Integrity (little ss-breaks, shearing, protection against nucleases) Quantity (high yield, especially with little sample …

X ray crystallography

English You can get amazed by the complexity of protein structures, but have you ever wonder how scientists get those images? In this post we're trying to simplify the main procedure used to determine protein structure: x ray crystallography. What is X ray crystallography? This is a technique used for getting the tridimensional structure of …

PAS

English What is PAS? PAS is a stain for detecting molecules containing a high amount of carbohydrates. The name stands for Periodic Acid Schiff since the two required reagents are the periodic acid and the Schiff's reagent. Principles The hydroxyl groups (-OH) of the carbohydrate molecule are oxidized by the periodic acid. The previous oxidation …

RT-PCR

  English What is RT-PCR? RT-PCR means “Reverse transcription polymerase chain reaction”, since it is a PCR variant that uses an enzyme that transcribes RNA to DNA. Thus, the starting point is RNA (messenger, ribosomal, transfer or micro RNA) and the final point is always cDNA, which will be amplified. Advantages and uses of RT-PCR …

Cornea transplant

Find here the steps we follow to check the cornea before it is transplanted

H-E principles

We use it everyday. Do not forget its principles!

Gene Expression Profiles for Early Stage Lung Adenocarcinoma

Español De acuerdo con la última guía ESMO para el manejo de carcinoma pulmonar de célula no pequeña (CPCNP) en estadios tempranos, se debe ofrecer quimioterapia adyuvante a todos los pacientes en estadio II, III. No debe valorarse en pacientes con enfermedad IA y, podría ser útil en pacientes con estadiaje IB y factores de …

Endopredict for early stage breast cancer

English Endopredict is a gene expression profile for ER (estrogen receptor) +, HER2-, early stage breast cancer. It studies the differential levels of expression of 11 genes. 3 of them taken as reference while the remaining 8 are genes of interest related to breast cancer development. 1 The figure bellow summarizes the processes to which …

Inhibition of the LDHA enzyme

English An attractive strategy for the treatment of Primary Hyperoxaluria could be the inhibition of the LDHA enzyme. But, how could we know if a compound is an inhibitor? or how good is it? For that purpose, a method has been developed that consists in the measurement of the absorbance of NADH at 340 nm, the following …

Docking

English When you are trying to study an interaction between a small molecule and a protein, Docking studies are very useful. They use bioinformatic tools to predict (with more or less accuracy) how a ligand would interact with a receptor, in my case with an enzyme. These tools have an important role to guide the …